Cathepsin G Activity Assay (BN00426)

SKU:
BN00426
  • Cell Health Assays Cathepsin G Activity Assay BN00426
  • Cell Health Assays Cathepsin G Activity Assay BN00426
  • Cell Health Assays Cathepsin G Activity Assay BN00426
€667

Description

Technical Manual MSDS

Cathepsin G Activity Assay

Assay Genie’s Cathepsin G Activity Assay provides a quick and easy method for monitoring Cathepsin G. In the assay Cathepsin G cleaves the substrate to release the dye group pNA (4-Nitroaniline), which can be detect at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped.

Cathepsin G Activity Assay Kit Data

Assay Information Description
Product Name: Cathepsin G Activity Assay
Product SKU: BN00426
Size: 100 assays
Application: Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.
Detection Method: Colorimetric (Absorbance 405 nm)
Sample Type: Serum, Tissue Extracts & Cell Lysate
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel Pack
Usage: For research use only. Not for use in humans.

Cathepsin G Assay Kit Components

Component Quantity
Assay Buffer 25 ml
Substrate 200 µl
Cathepsin G Inhibitor 20 µl
pNA Standard (0.1 M) 20 µl
Positive Control 1 vial

Product Images

Figure: a) Relative Activity Curve b) pNA Standard Curve c) Histogram of Positive Control with and without Inhibitor

Cathepsin G Background

Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

View AllClose

Additional Information

Product type:
Cellular Assay
Detection Method:
Colorimetric
View AllClose