Cathepsin G Activity Colorimetric Assay Kit (BN00426)

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BN00426
  • Cathepsin G Activity Colorimetric Assay Kit (BN00426)
  • Cathepsin G Activity Colorimetric Assay Kit (BN00426)
  • Cathepsin G Activity Colorimetric Assay Kit (BN00426)
€667

Description

ELISA Kit Technical ManualMSDS

Cathepsin G Activity Colorimetric Assay Kit

Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In Assay Genie's Cathepasin G Activity assay Kit, Cathepsin G will cleave the substrate and release the dye group, pNA (4-Nitroaniline), which can be detect at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable for the activity of Cathepsin G.

Figure: a) Relative Activity Curve b) pNA Standard Curve c) Histogram of Positive Control with and without Inhibitor

Key Information Description

Product SKU

BN00426

Size

100 assays

Detection Method

Absorbance (405 nm)

Species Reactivity

Mammalian

Applications

Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.

Features and Benefits

  • Simple one-step procedure; takes only 1 hours
  • Fast, robust and convenient
  • Cathepsin-G assay is straightforward, and can be adapted to 96-well plate assays.

Kit Components

  • Assay Buffer
  • Substrate
  • Cathepsin G Inhibitor
  • pNA Standard (0.1 M)
  • Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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