Cathepsin G Inhibitor Screening Kit (Colorimetric) (BN00442)

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SKU:
BN00442
€732

Description

ELISA Kit Technical ManualMSDS

Cathepsin G Inhibitor Screening Kit (Colorimetric)

Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. Assay Genie's Cathepsin G Inhibitor Screening Kit utilizes the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.

Figure: Inhibition of Cathepsin G activity by CTSG Inhibitor. Assay was performed following kit protocol.

Key Information Description

Product SKU

BN00442

Size

100 assays

Detection Method

Absorbance (OD 405 nm)

Applications

Screen/study/characterize potential inhibitors of Cathepsin G

Features and Benefits

  • Convenient & Rapid
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin G
  • Cathepsin G inhibitor is included in the kit

Kit Components

  • CTSG Assay Buffer
  • Cathepsin G (human)
  • CTSG Substrate
  • CTSG Inhibitor (0.5 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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