Cathepsin K Inhibitor Screening Kit (Fluorometric) (BN00430)

Product type:
Cellular Assay


ELISA Kit Technical ManualMSDS

Cathepsin K Inhibitor Screening Kit (Fluorometric)

Cathepsin K (CTSK, EC, a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin. Assay Genie's Cathepsin K Inhibitor Screening Kit utilizes the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.

Figure: Inhibition of Cathepsin K activity by CTSK Inhibitor. Assay was performed following kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em = 400/505 nm)


Screen/study/characterize potential inhibitors of Cathepsin K.

Features and Benefits

  • Simple & convenient
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin K
  • Cathepsin K inhibitor, FF-FMK is included in the kit

Kit Components

  • CTSK Reaction Buffer
  • CTSK Reagent
  • Cathepsin K (Human)
  • CTSK Substrate, Ac-LR-AFC (10 mM)
  • CTSK Inhibitor (FF-FMK, 1 mM)

Storage Conditions


Shipping Conditions

Dry Ice


For Research Use Only! Not For Use in Humans.