Description
Cathepsin K Inhibitor Screening Kit (Fluorometric)
Cathepsin K (CTSK, EC 3.4.22.38), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin. Assay Genie's Cathepsin K Inhibitor Screening Kit utilizes the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.
Figure: Inhibition of Cathepsin K activity by CTSK Inhibitor. Assay was performed following kit protocol.
Key Information | Description |
Product SKU | BN00430 |
Size | 100 assays |
Detection Method | Fluorescence (Ex/Em = 400/505 nm) |
Applications | Screen/study/characterize potential inhibitors of Cathepsin K. |
Features and Benefits |
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Kit Components |
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Storage Conditions | -80°C |
Shipping Conditions | Dry Ice |
USAGE | For Research Use Only! Not For Use in Humans. |