Cathepsin S Inhibitor Screening Kit (Fluorometric) (BN00429)

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ELISA Kit Technical ManualMSDS

Cathepsin S Inhibitor Screening Kit (Fluorometric)

Cathepsin S (CTSS, EC is a lysosomal cysteine proteinase that is suggested to participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. Assay Genie's Cathepsin S Inhibitor Screening Kit utilizes the ability of Cathepsin S to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin S inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive, and rapid tool to screen the potential inhibitors of Cathepsin S.

Figure: Inhibition of Cathepsin S activity by CTSS Inhibitor. Assay was performed following kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em = 400/505 nm)


Screen/study/characterize potential inhibitors of Cathepsin S.

Features and Benefits

  • Simple & convenient
  • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin S
  • Cathepsin S inhibitor, Z-FF-FMK is included in the kit

Kit Components

  • CTSS Reaction Buffer
  • Cathepsin S (human)
  • CTSS Substrate, Z-VVR-AFC (10 mM)
  • CTSS Inhibitor (Z-FF-FMK, 1 mM)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

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