CDC42BPB Antibody (PACO57052)
- SKU:
- PACO57052
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | CDC42BPB Antibody (PACO57052) |
| Antibody SKU: | PACO57052 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Serine/threonine-protein kinase MRCK β protein (1609-1711AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO57052 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57052 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. |
| Synonyms: | Serine/threonine-protein kinase MRCK β (EC 2.7.11.1) (CDC42-binding protein kinase beta) (CDC42BP-beta) (DMPK-like beta) (Myotonic dystrophy kinase-related CDC42-binding kinase beta) (MRCK beta) (Myotonic dystrophy protein kinase-like beta), CDC42BPB, KIAA1124 |
| UniProt Protein Function: | MRCKB: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; GEK subfamilyChromosomal Location of Human Ortholog: 14q32.3Cellular Component: actomyosin; cytoplasm; cytoskeleton; intercellular junction; leading edge; plasma membraneMolecular Function: ATP binding; magnesium ion binding; protein kinase activity; protein serine/threonine kinase activity; Rho GTPase bindingBiological Process: actin cytoskeleton reorganization; actomyosin structure organization and biogenesis; cell migration; cytoskeleton organization and biogenesis; establishment and/or maintenance of cell polarity; protein amino acid phosphorylation; signal transduction |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y5S2 |
| NCBI GenInfo Identifier: | 92090617 |
| NCBI Gene ID: | 9578 |
| NCBI Accession: | Q9Y5S2.2 |
| UniProt Secondary Accession: | Q9Y5S2,Q2L7A5, Q86TJ1, Q9ULU5, A9JR72 |
| UniProt Related Accession: | Q9Y5S2 |
| Molecular Weight: | 194,315 Da |
| NCBI Full Name: | Serine/threonine-protein kinase MRCK beta |
| NCBI Synonym Full Names: | CDC42 binding protein kinase beta |
| NCBI Official Symbol: | CDC42BPBÂ Â |
| NCBI Official Synonym Symbols: | MRCKBÂ Â |
| NCBI Protein Information: | serine/threonine-protein kinase MRCK beta |
| UniProt Protein Name: | Serine/threonine-protein kinase MRCK beta |
| UniProt Synonym Protein Names: | CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like beta |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | CDC42BPBÂ Â |
| UniProt Entry Name: | MRCKB_HUMAN |
| Antibodies |
| CDC42BPB Antibody (PACO01083) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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