Cell Cycle Analysis Kit (BN01094)

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BN01094
  • Cell Cycle Analysis Kit (BN01094)
  • Cell Cycle Analysis Kit (BN01094)
£465.36

Description

ELISA Kit Technical ManualMSDS

Cell Cycle Analysis Kit

Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell Cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase). Assay Genie's Cell Cycle Analysis Kit provides a quick and easy method to detect the number of cells in a cell population, which are at a specific stage of the cell cycle. Our kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. Our method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Figure: 3T3 Cell Cycle Analysis: 3T3 cells were seeded at 2 x 10 5 cells/well into a 6-well tissue culture plate with 10% FBS culture medium on day 1. On day 2, medium was removed and cells synchronized with 0.1% FBS culture medium for 24 hr. On day 3, 3T3 cells were incubated in culture medium with either 0.1% FBS, 10% FBS, or 10% FBS containing 20 µM of SKPin C1 (G1 or G2/M phase blocker) for an additional 24 hr. On day 4, cells were harvested, fixed and stained with the Cell Cycle Analysis Kit according to the protocol. Fluorescence intensity was detected and recorded on a BD Flow Cytometer in FL-2 channel. A. Cell cycle analysis of 3T3 cells in 10% FBS culture medium; B. Cell cycle analysis of 3T3 cells in 10% FBS culture medium containing 20 µM of SKPin C1.

Key Information Description

Product SKU

BN01094

Size

100 assays

Detection Method

Flow cytometry (Ex 488 nm)

Species Reactivity

Mammalian

Applications

  • Analysis of cell cycle regulation in response to growth factors, cytokines, mitogens, and nutrients, etc.
  • Monitoring of cell cycle progression
  • Study effects of drugs which affect cell growth and division

Features and Benefits

  • Highly sensitive flow cytometry-based method to detect and monitor cells at various stages of the cell cycle
  • Simple & High throughput-adaptable
  • Easy tool for screening compounds that affect cell growth and division

Kit Components

  • 10X Cell Cycle Assay Buffer
  • Enzyme A Solution
  • Nuclear Dye

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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