Ceruloplasmin Activity Colorimetric Assay Kit (BN00893)

SKU:
BN00893
  • Metabolism Assays Ceruloplasmin Activity Colorimetric Assay Kit BN00893
  • Metabolism Assays Ceruloplasmin Activity Colorimetric Assay Kit BN00893
€631

Description

ELISA Kit Technical ManualMSDS

Ceruloplasmin Activity Colorimetric Assay Kit

Ceruloplasmin is a copper containing protein found primarily in the blood. It carries approximately 70% of the total copper present in the blood. Ceruloplasmin exhibits a weak oxidase activity, which is a more accurate method of assessing ceruloplasmin in serum than immunodiffusion or other non-enzymatic assays. Normal Ceruloplasmin levels are generally 1-4 µM (15-60 mg/dl), equivalent to approximately 50-150 mU/ml. Elevated amounts of serum ceruloplasmin are found in pregnancy, cancer, rheumatoid arthritis and in several mental conditions such as Alzheimer's, schizophrenia and OCD. Abnormally low amounts are found in Wilson's and Menkes' Diseases and in several other rare conditions. Assay Genie's Ceruloplasmin Activity Assay Kit utilizes an oxidase substrate, which gives an intensely colored product upon oxidation. The kit is fast, easy to use and suitable for high throughput applications and can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.

Figure: (a) Standard Curve of chemically oxidized substrate. (b) Ceruloplasmin Activity Assay: Kinetic curves obtained from varying sample volumes of human serum. Assays were performed following kit protocol. The Standard Curve slope (for the points below 1.0 OD) is 0.0214 (a) & the kinetic slope for a 10 μl
sample of an Ammonium Sulfate precipitate of frozen pooled human serum is 0.0189 (b). Serum Ceruloplasmin Activity = 0.0189 (OD/min)/0.0214 (OD/nmol)/0.01ml X 2 = 176 mU/ml.

Key Information Description

Product SKU

BN00893

Size

100 assays

Detection Method

Absorbance (OD 560 nm)

Applications

Measurement of ceruloplasmin activity in serum

Features and Benefits

  • Simple, highly sensitive, and high-throughput suitable.
  • The assay kit can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.

Kit Components

  • Ceruloplasmin Assay Buffer
  • Ammonium Sulfate, saturated (~4.1M)
  • Ceruloplasmin Substrate
  • Oxidizer (100 mM)
  • Stabilizer

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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