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Anti-CHIT1 Antibody (CAB2015)

SKU:
CAB2015
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-CHIT1 Antibody CAB2015
  • Metabolism Antibodies 2 Anti-CHIT1 Antibody CAB2015
€139 - €419
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Description

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Antibody Name:Anti-CHIT1 Antibody
Antibody SKU:CAB2015
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:U-251MG, 293T, Mouse spleen, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SWEK VTGH NSPL YKRQ EESG AAAS LNVD AAVQ QWLQ KGTP ASKL ILGM PTYG RSFT LASS SDTR VGAP ATGS GTPG PFTK EGGM LAYY EVCS WKGA TKQR IQDQ KVPY IFRD NQWV GFDD VESF KTKV SYLK QKGL GGAM VWAL DLDD FAGF SCNQ GRYP LIQT LRQE LSLP YLPS GTPE LEVP KPGQ PSEP EHGP SPGQ DTFC QGKA DGLY PNPR ERSS FYSC AAGR LFQQ SCPT GLVF SNSC KCCT WN
Gene ID:1118
Uniprot:Q13231
Cellular Location:Lysosome, Secreted
Calculated MW:43kDa/48kDa/49kDa/51kDa
Observed MW:52kDa
Synonyms:CHIT1, CHI3, CHIT, CHITD
Background:Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; lysosome

Molecular Function:endochitinase activity; chitin binding; chitinase activity

Biological Process: polysaccharide catabolic process; response to bacterium; immune response; chitin catabolic process

Disease: Chitotriosidase Deficiency

NCBI Summary:Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q13231
NCBI GenInfo Identifier:37999493
NCBI Gene ID:1118
NCBI Accession:Q13231.1
UniProt Related Accession:Q13231
Molecular Weight:
NCBI Full Name:Chitotriosidase-1
NCBI Synonym Full Names:chitinase 1
NCBI Official Symbol:CHIT1  
NCBI Official Synonym Symbols:CHI3; CHIT; CHITD  
NCBI Protein Information:chitotriosidase-1
UniProt Protein Name:Chitotriosidase-1
UniProt Synonym Protein Names:Chitinase-1
Protein Family:Chitotriosidase
UniProt Gene Name:CHIT1  
UniProt Entry Name:CHIT1_HUMAN
Anti-CHIT1 Antibody (CAB2015)Western blot analysis of extracts of various cell lines, using CHIT1 antibody (CAB2015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CHIT1 Antibody (CAB2015)Immunohistochemistry of paraffin-embedded human normal stomach using CHIT1 antibody (CAB2015) at dilution of 1:200 (40x lens).

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