Anti-CHMP1A Antibody (CAB11621)

Antibody Name:	Anti-CHMP1A Antibody
Antibody SKU:	CAB11621
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human CHMP1A (NP_002759.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, LO2, HT-29, 22Rv1, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human CHMP1A (NP_002759.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDT LFQL KFTA KQLE KLAK KAEK DSKA EQAK VKKA LLQK NVEC ARVY AENA IRKK NEGV NWLR MASR VDAV ASKV QTAV TMKG VTKN MAQV TKAL DKAL STMD LQKV SSVM DRFE QQVQ NLDV HTSV MEDS MSSA TTLT TPQE QVDS LIMQ IAEE NGLE VLDQ LSQL PEGA SAVG ESSV RSQE DQLS RRLA ALRN
Gene ID:	5119
Uniprot:	Q9HD42
Cellular Location:	Cytoplasm, Endosome membrane, Nucleus matrix, Peripheral membrane protein
Calculated MW:	7kDa/21kDa
Observed MW:	35kDa

Synonyms:

CHMP1A, CHMP1, PCH8, PCOLN3, PRSM1, VPS46-1, VPS46A

Background:

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene.

UniProt Protein Function:	CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Protease Chromosomal Location of Human Ortholog: 16q24.3 Cellular Component: condensed nuclear chromosome; early endosome; endomembrane system; microtubule organizing center; nuclear matrix Molecular Function:identical protein binding; metallopeptidase activity; protein binding; protein domain specific binding; protein homodimerization activity; zinc ion binding Biological Process: cell division; cell separation during cytokinesis; gene silencing; mitotic chromosome condensation; mitotic metaphase plate congression; negative regulation of transcription by glucose; negative regulation of transcription, DNA-dependent; nuclear organization and biogenesis; vesicle-mediated transport Disease: Pontocerebellar Hypoplasia, Type 8
NCBI Summary:	This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
UniProt Code:	Q9HD42
NCBI GenInfo Identifier:	62510514
NCBI Gene ID:	5119
NCBI Accession:	Q9HD42.1
UniProt Secondary Accession:	Q9HD42,Q14468, Q15779, Q96G31, A2RU09,
UniProt Related Accession:	Q9HD42
Molecular Weight:	22kDa
NCBI Full Name:	Charged multivesicular body protein 1a
NCBI Synonym Full Names:	charged multivesicular body protein 1A
NCBI Official Symbol:	CHMP1A
NCBI Official Synonym Symbols:	PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
NCBI Protein Information:	charged multivesicular body protein 1a
UniProt Protein Name:	Charged multivesicular body protein 1a
UniProt Synonym Protein Names:	Chromatin-modifying protein 1a; CHMP1a; Vacuolar protein sorting-associated protein 46-1; Vps46-1; hVps46-1
Protein Family:	Charged multivesicular body protein
UniProt Gene Name:	CHMP1A

Western blot analysis of extracts of various cell lines, using CHMP1A antibody (CAB11621) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

CHMP1A Antibody (PACO08408)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)