Choline Kinase (ChoK) Activity Assay Kit (Fluorometric) (BN00713)

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BN00713
  • Choline Kinase (ChoK) Activity Assay Kit (Fluorometric) (BN00713)
  • Choline Kinase (ChoK) Activity Assay Kit (Fluorometric) (BN00713)
  • Choline Kinase (ChoK) Activity Assay Kit (Fluorometric) (BN00713)
€1,016

Description

ELISA Kit Technical ManualMSDS

Choline Kinase (ChoK) Activity Assay Kit (Fluorometric)

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. Assay Genie provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.

Figure (A). ADP Standard Curve. (B). Kinetic reaction rate of ChoK with and without choline substrate in the assay. (C). Activity of ChoK determined by the assay in different samples.

Key Information Description

Product SKU

BN00713

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (Ex/Em 535/587 nm)
  • Species reactivity- Eukaryotes
  • Applications- The kit detects 0.5 µM choline kinase.

Detection Method

Fluorescence (Ex/Em= 535/587 nm)

Species Reactivity

Eukaryotes

Applications

Measurement of choline kinase activity in different cell lines and tissues

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • Assay Buffer
  • Substrate
  • Detection Mix I
  • Detection Mix II
  • ATP
  • Probe
  • ADP Standard (1 µmol)
  • Human ChoK

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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