Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions) (BN00088)

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BN00088
€1,135

Description

ELISA Kit Technical ManualMSDS

Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. Together with RNAs, genomic single or double-stranded DNA and mitochondrial DNA have been recently detected in exosomes and microvesicles. In particular, the majority of the double-stranded DNA seems to be associated with tumor derived exosomes and can be an important new source of biomarkers for tumor detection.

Isolation and purification of circulating and Exosome-associated DNA kit combines the ability of Isolation Component to isolate exosomes from a wide range of biofluids (plasma and serum) with a user-friendly system of DNA purification. Isolated exosomes are lysed with the appropriate lysis buffer and exosome DNA is purified by and exosome DNA is purified by spin columns and optimized buffers with a fast turnaround time. In addition, this kit provides lyophilized exosomes to be used as quality controls for exosome capture and DNA extraction. The procedure takes about 1 hour and 45 minutes for yielding purified genomic DNA. Furthermore, this kit provides lyophilized exosomes to be used as quality controls for exosome capture and DNA extraction. Exosome standards for positive control are also included in the kit. All our kits guarantee high efficiency isolation of circulating and Exosome-associated genomic DNA than similar products.

Figure: Amplification of beta-actin from exosome-derived DNA. Exosomes were isolated from serum with our without artificial spike (A375-derived exosomes) using Exosome Purification solution and treated (or not) with DNAse I. DNA was extracted with the AssayGenie kit and competitor and beta actin was amplified by qPCR.

Key Information Description

Detection Method

Isolation and purification of circulating and Exosome-associated genomic DNA from plasma and serum

Size Options

  • 20 Reactions
  • 40 Reactions

Species Reactivity

Human plasma, serum

Applications

Isolation and purification of circulating and Exosome-associated genomic DNA from plasma and serum

Features and Benefits

Direct exosome capture and DNA purification from plasma and serum without time consuming purification steps.

Kit Components

  • Exosome Lysis buffer
  • Proteinase K
  • Washing buffer 1
  • Washing buffer 2
  • Elution buffer
  • Columns
  • RNase free elution tubes (1.5 ml)
  • Lyophilized Exosome Standard (Plasma/Serum)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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