Anti-CLDN5 Antibody (CAB10207)

Antibody Name:	Anti-CLDN5 Antibody
Antibody SKU:	CAB10207
Antibody Size:	20uL, 50uL, 100uL
Application:	IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-303 of human CLDN5 (NP_001124333.1).

Application:	IF
Recommended Dilution:	IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-303 of human CLDN5 (NP_001124333.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	REFY DPSV PVSQ KYEL GAAL YIGW AATA LLMV GGCL LCCG AWVC TGRP DLSF PVKY SAPR RPTA TGDY DKKN YV
Gene ID:	7122
Uniprot:	O00501
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, tight junction
Calculated MW:	23kDa
Observed MW:	27kDa

Synonyms:

CLDN5, AWAL, BEC1, CPETRL1, TMVCF, claudin-5

Background:

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.
UniProt Protein Details:	Protein type:Cytoskeletal; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: apicolateral plasma membrane; cortical actin cytoskeleton; integral to membrane; intercellular junction; plasma membrane; tight junction Molecular Function:identical protein binding Biological Process: calcium-independent cell-cell adhesion; intercellular junction assembly; learning; palate development; transforming growth factor beta receptor signaling pathway
NCBI Summary:	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	O00501
NCBI GenInfo Identifier:	6685271
NCBI Gene ID:	7122
NCBI Accession:	O00501.1
UniProt Secondary Accession:	O00501,Q53XW2, Q8WUW3, B3KS11,
UniProt Related Accession:	O00501
Molecular Weight:	23kDa
NCBI Full Name:	Claudin-5
NCBI Synonym Full Names:	claudin 5
NCBI Official Symbol:	CLDN5
NCBI Official Synonym Symbols:	AWAL; BEC1; TMVCF; CPETRL1
NCBI Protein Information:	claudin-5
UniProt Protein Name:	Claudin-5
UniProt Synonym Protein Names:	Transmembrane protein deleted in VCFS; TMDVCF
Protein Family:	Claudin
UniProt Gene Name:	CLDN5

	Immunofluorescence analysis of NIH/3T3 cells using CLDN5 antibody (CAB10207) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using CLDN5 antibody (CAB10207) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Phospho-CLDN5 (Y217) Antibody (PACO05216)	Human CLDN5 / Claudin 5 ELISA Kit
CLDN5 Antibody (PACO05217)	Claudin 5 Colorimetric Cell-Based ELISA Kit
CLDN5 Antibody (PACO06353)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)