Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit (BN00630)

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SKU:
BN00630
  • Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit (BN00630)
  • Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit (BN00630)
€589

Description

ELISA Kit Technical ManualMSDS

Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. Assay Genie has developed an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with GenieRed Probe to generate color (= 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.

Figure 1: Standard Graphs obtained from following the Kit Protocol.

Key Information Description

Product SKU

BN00630

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.

Features and Benefits

  • Simple procedure; takes less than 1 hour to complete the assay
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable

Kit Components

  • CoA Assay Buffer
  • GenieRed Probe
  • DMSO (anhydrous)
  • Conversion Enzyme Mix
  • CoA Substrate
  • Acyl CoA Developer
  • CoA Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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