COVID-19 Spike Protein ELISA Kit

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ELISA Kit Technical ManualELISA Kit Technical ManualMSDS

COVID-19 Spike Protein ELISA Kit 

The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, plasma (EDTA, citrate, heparin).

This COVID-19 Spike Protein ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SARS-COV-2 Spike Protein in the sample, this COVID-19 Spike Protein ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus COVID-19 Spike Protein concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Information Description

Product Name

COVID-19 Spike Protein ELISA Kit

SKU Number





100 uL

Kit Components

  • ELISA Microplate(Dismountable)
  • Lyophilized Standard
  • Sample/Standard Dilution Buffer
  • Biotin-labeled Antibody(Concentrated)
  • Assay Dilution
  • Antibody Dilution Buffer
  • HRP-Streptavidin Conjugate (SABC)
  • SABC Dilution Buffer
  • TMB Substrate A
  • TMB Substrate B
  • Stop Solution
  • Wash Buffer(25X)
  • Plate Sealer


Microplate Reader






390 fg/ml


Coronavirus(COVID-19,SARS-CoV-2, 2019-nCoV, Novel Coronavirus Spike Glycoprotein,RBD, SARSCoV-2 S1).

Standard Curve Range


Storage Instructions

2-8°C for 6 months


For quantitative detection of 2019 nCoV (S) in serum, plasma, tissue homogenates, cell culture supernatant, cell culture lysate and other biological fluids (throat swab, nose swab).

Safety Notes

1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended
2. After opening and before using, keep plate dry.
3. Before using the kit, spin tubes and bring down all components to the bottom of tubes.
4. Storage TMB reagents avoid light.
5. Washing process is very important, not fully wash easily cause a false positive and high background.
6. Duplicate well assay is recommended for both standard and sample testing.
7. Don’t let microplate dry at the assay, for dry plate will inactivate active components on plate.
8. Don’t reuse tips and tubes to avoid cross contamination.
9. Avoid using the reagents from different batches together.

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