Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (BN00779)

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BN00779
  • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (BN00779)
  • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (BN00779)
  • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (BN00779)
  • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (BN00779)
€487

Description

ELISA Kit Technical ManualMSDS

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by non-steroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain. Assay Genie's COX Activity Assay Kit provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. Detection limit: 6 /mg.

Figure: (a) Resorufin Standard Curve. (b) Measurement of Cox-1 Positive Control (PC) activity. (c) Detection of endogenous Cox I activity in J774 cell lysate (6 μg) and rat liver homogenate (210 μg). (d) Detection of endogenous Cox II activity in J774 cell lysate (7 μg) stimulated with or without 100 ng/ml LPS and 100 ng/ml murine IFN-gamma. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00779

Size

100 assays

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Applications

Measurement of COX activity in various biological samples and purified/crude enzyme preparations

Features and Benefits

  • Simple, rapid & convenient assay to measure both COX-1 and COX-2 activity
  • High-throughput adaptable
  • Includes COX-1 inhibitor, SC560 & COX-2 Inhibitor, Celecoxib

Kit Components

  • COX Assay Buffer
  • COX Probe (in DMSO)
  • COX Cofactor (in DMSO)
  • Arachidonic Acid
  • NaOH
  • COX-1, Ovine
  • SC560, COX-1 inhibitor (in DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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