Cyclooxygenase Activity Assay (BN00779)

SKU:
BN00779
Product type:
Cellular Assay
Detection Method:
Fluorometric
  • Cell Biology Assays Cyclooxygenase Activity Assay BN00779
  • Cell Biology Assays Cyclooxygenase Activity Assay BN00779
  • Cell Biology Assays Cyclooxygenase Activity Assay BN00779
  • Cell Biology Assays Cyclooxygenase Activity Assay BN00779
€487

Description

Technical Manual MSDS

Cyclooxygenase Activity Assay

Assay Genie's Cyclooxygenase Activity Assay provides a sensitive and high-throughput method to detect the peroxidase activity of Cyclooxygenase (COX) in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. The Cyclooxygenase Activity Assay has a detection limit of 6 µU/mg.

Assay Information Description
Product Name: Cyclooxygenase Activity Assay
Product SKU: BN00779
Size: 100 assays
Application: Measurement of COX activity in various biological samples and purified/crude enzyme preparations.
Detection Method: Fluorometric (Ex/Em 535/587 nm)
Sensitivity: 6 µU/mg
Sample Type:
  • Adherent and suspension cells
  • Animal tissues
  • Purified enzyme
Storage: Store the kit at -20°C, protect from light
Shipping Conditions: Gel pack
Usage: For research use only. Not for use in humans.

Cyclooxygenase Activity Assay Kit Components

Component Quantity
COX Assay Buffer 25 ml
COX Probe (in DMSO) 200 µl
COX Cofactor (in DMSO) 20 µl
Arachidonic Acid 50 µl
NaOH 500 µl
COX-1 Positive Control 1 vial
Resorufin Standard (5 mM, in DMSO) 50 µl
SC560 (COX-1 inhibitor in DMSO) 100 µl
Celecoxib (COX-2 inhibitor in DMSO) 100 µl

Product Images

Figure: (a) Resorufin Standard Curve. (b) Measurement of Cox-1 Positive Control (PC) activity. (c) Detection of endogenous Cox I activity in J774 cell lysate (6 μg) and rat liver homogenate (210 μg). (d) Detection of endogenous Cox II activity in J774 cell lysate (7 μg) stimulated with or without 100 ng/ml LPS and 100 ng/ml murine IFN-gamma. Assays were performed following the kit protocol.

Cyclooxygenase Background

Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.