Anti-CYR61 Antibody (CAB1111)

Antibody Name:	Anti-CYR61 Antibody
Antibody SKU:	CAB1111
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CYR61

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, OVCAR3, HeLa, C2C12, Mouse heart, Mouse lung, Rat lung, Rat spleen

Immunogen:	Recombinant protein of human CYR61
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3491
Uniprot:	O00622
Cellular Location:	Secreted
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:	CYR61, CCN1, GIG1, IGFBP10
Background:	The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation.

UniProt Protein Function:	CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1p22.3 Cellular Component: extracellular matrix; proteinaceous extracellular matrix Molecular Function:heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding Biological Process: anatomical structure morphogenesis; extracellular matrix organization and biogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of cell growth; cell adhesion; positive regulation of BMP signaling pathway; positive regulation of cell migration; negative regulation of apoptosis
NCBI Summary:	The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]
UniProt Code:	O00622
NCBI GenInfo Identifier:	3023601
NCBI Gene ID:	3491
NCBI Accession:	O00622.1
UniProt Secondary Accession:	O00622,O14934, O43775, Q9BZL7,
UniProt Related Accession:	O00622
Molecular Weight:
NCBI Full Name:	Protein CYR61
NCBI Synonym Full Names:	cysteine-rich, angiogenic inducer, 61
NCBI Official Symbol:	CYR61
NCBI Official Synonym Symbols:	CCN1; GIG1; IGFBP10
NCBI Protein Information:	protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
UniProt Protein Name:	Protein CYR61
UniProt Synonym Protein Names:	CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1
Protein Family:	Protein
UniProt Gene Name:	CYR61
UniProt Entry Name:	CYR61_HUMAN

	Western blot analysis of extracts of various cell lines, using CYR61 antibody (CAB1111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of L929 cells using CYR61 antibody (CAB1111) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) CLIA Kit (HUES00738)

Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit (HUES02342)

Human Protein CYR61 (CYR61) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)