Cystathionine Beta Synthase Activity Assay Kit (Fluorometric) (BN01155)

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BN01155
  • Cystathionine Beta Synthase Activity Assay Kit (Fluorometric) (BN01155)
  • Cystathionine Beta Synthase Activity Assay Kit (Fluorometric) (BN01155)
  • Cystathionine Beta Synthase Activity Assay Kit (Fluorometric) (BN01155)
€708

Description

ELISA Kit Technical ManualMSDS

Cystathionine Beta Synthase Activity Assay Kit (Fluorometric)

Cystathionine Beta Synthase (EC 4.2.1.22, CBetaS) is a PLP-dependent enzyme that catalyzes the formation of H2S and cystathionine when using cysteine and homocysteine as substrates. Known for its role in human sulfur metabolism, mutations in the gene encoding CBetaS can result in high concentrations of homocysteine in plasma. Elevated circulating concentrations of homocysteine result in genetic disorders including Homocysteinuria and Down syndrome. Specifically, Homocysteinuria is an autosomal recessive disease with clinical manifestations including mental retardation, thromboembolism and connective tissue defects. Hydrogen sulfide, CBetaS product, is an important gaseous mediator, like nitric oxide, that has significant effects on the immunological, neurological, cardiovascular and pulmonary systems of mammals. Assay Genie's Cystathione Beta Synthase Assay Kit utilizes cysteine and homocysteine as substrates to produce H2S. Hydrogen sulfide reacts with the azido-functional group of the fluorescent probe yielding a fluorescent amino group (Ex/Em = 368/460 nm). The assay is highly sensitive, has a simple easy-to-follow protocol, and can detect as low as 1.45 mU of CBetaS activity.

Figure: (a) CβS Standard Curve, (b) CβS activity in Liver Lysate (20 µg) Positive Control (1.5 µg) (c) CβS specific activity in HepG2 cell lysate and Liver lysate. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN01155

Size

100 assays

Detection Method

Fluorescence (Ex/Em 368/460 nm)

Species Reactivity

Eukaryotes

Applications

Rapid assessment of native/recombinant CBetaS and CBetaS activity in mammalian samples

Features and Benefits

  • Simple, convenient, highly sensitive
  • Fluorescent assay enables easy determination of CBetaS activity in a variety of biological samples
  • Kit includes a stable CBetaS positive control

Kit Components

  • CBetaS Assay Buffer
  • CBetaS Probe (in DMSO)
  • CBetaS Substrate
  • Cofactor 1
  • Cofactor 2
  • Reducing Agent
  • AMC Standard (in DMSO, 1 mM)
  • CBetaS Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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