Cytochrome Oxidase Activity Colorimetric Assay Kit (BN00545)

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SKU:
BN00545
€619

Description

ELISA Kit Technical ManualMSDS

Cytochrome Oxidase Activity Colorimetric Assay Kit

Cytochrome c Oxidase (EC 1.9.3.1) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP. Cytochrome Oxidase Activity Assay Kit is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm.

Figure: Cytochrome Oxidase Activity: Purified mitochondria (Samples) were used to calculate a decrease in OD at 550 nm (Conc. 0.625-5 µg/reaction). In Blank, no change in the OD was observed. Rate is calculated by subtracting the initial OD reading from the final OD, t1 and t2 represents linear rate of reaction.

Key Information Description

Product SKU

BN00545

Size

100 assays

Applications

  • Simple and rapid measurement of cytochrome oxidase enzymatic activity in 96-well plate format.
  • Useful for mitochondrial respiration studies, assembly of the complexes, mitochondria outer membrane integrity.

Features and Benefits

  • Simple & Rapid
  • High-throughput adaptable
  • The kit provides unique formulation of buffers & reagents to measure cytochrome oxidase activity

Kit Components

  • Cytochrome Oxidase Assay Buffer
  • Enzyme Dilution Buffer
  • 1 M DTT
  • Cytochrome c
  • 96-well Plate

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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