In Eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and alpha-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of alpha-ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
How does our D-2-Hydroxyglutarate Assay Kit work
Assay Genie's D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to alpha-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 uM in various samples.
D-2-Hydroxyglutarate Assay Kit Information:
|Detection Method:||Absorbance (OD= 450 nm).|
|Applications:||Measurement of D2HG level in various cell/tissues/biological fluids.|
|Features & Benefits:||Simple, fast, HTP adaptable and convenient assay, Can measure D2HG levels as low as 10 uM in various samples|
|Kit Components:||D2HG Assay Buffer, D2HG Enzyme, D2HG Substrate Mix, D2HG Standard|
|Use:||For Research Use Only! Not For Use in Humans.|