Deproteinizing Sample Preparation Kit (BN01024)

SKU:
BN01024
€448

Description

ELISA Kit Technical ManualMSDS

Deproteinizing Sample Preparation Kit

The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc.The newly designed Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules. Using the kit, proteins are precipitated, excess PCA is removed, samples are neutralized. Samples prepared using this kit can be directly used in a wide variety of bioassays. The method is easy, convenient, and can be used for the preparation of a large number of samples.

Figure: Deproteinization of serum samples: Different amounts of PCA were used to deproteinize 400 μl of serum following the kit protocol. Protein concentration remaining in solution OD280 and sample pH was measured. Samples (neat serum; serum filtered through a 10kD MW cutoff; and PCA treated) were diluted 20 times for convenience of measuring OD at 280 nm. A separate aliquot of the same PCA treated samples was mixed 1:1 with a pH 7.75 assay buffer and the pH of the mixture determined.

Key Information Description

Product SKU

BN01024

Size

200 assays

Detection Method

Mammalian

Applications

Unique tool to prepare samples for the analysis of a variety of small molecules, proteins are precipitated, excess PCA is removed, samples are neutralized.

Features and Benefits

Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.

Kit Components

  • PCA
  • Neutralization Solution

Storage Conditions

RT

Shipping Conditions

RT

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Molecular Tools
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