Diamine Oxidase Activity Assay Kit (Fluorometric) (BN00729)

SKU:
BN00729
Product type:
Cellular Assay
  • Cell Biology Assays Diamine Oxidase Activity Assay Kit Fluorometric BN00729
  • Cell Biology Assays Diamine Oxidase Activity Assay Kit Fluorometric BN00729
  • Cell Biology Assays Diamine Oxidase Activity Assay Kit Fluorometric BN00729
€649

Description

ELISA Kit Technical ManualMSDS

Diamine Oxidase Activity Assay Kit (Fluorometric)

Diamine Oxidase (DAO, E.C. 1.4.3.22) is an important regulator of polyamine levels in human physiology. DAO metabolizes the oxidative conversion of biogenic polyamines such as putrescine and cadaverine into aldehydes. Biogenic amines are known regulators of important biological pathways such as cell growth and signal transduction, placing DAO in an important regulatory position in maintaining cell viability. Putrescine itself is also an activator of c-jun and c-fos expression, further linking this diamine with potential roles in cancer development. DAO is notably involved in regulating growth and differentiation of intestinal mucosa, bone marrow, and other rapidly proliferating tissue. In addition to its role in tissue growth, DAO activity is important in metabolism. Loss of diamine oxidase activity can lead to histamine intolerance and histamine poisoning. Assay Genie's Diamine Oxidase Activity Assay Kit provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmole/min of activity. In the assay, DAO converts the provided substrate, yielding an intermediate and H2O2. H2O2 is then utilized by the DAO Enzyme Mix to generate fluorescence (Ex/Em = 535/587 nm) from the DAO Probe.

Figure: a) H2O2 standard curve; (b) Kinetic measurement of DAO activity from Positive Control (c) Activity determination of intestinal tissue lysate. For this experiment, 100 mg rat intestine was used, following Diamine Oxidase Activity Assay Kit protocol and including protease inhibitor in the lysis. Lysate (7 µg) was assayed and specific activity was determined to be 1.30 nmol/min/mg lysate.

Key Information Description

Product SKU

BN00729

Size

100 assays

Kit Summary

  • Detection method: Fluorescence (Ex/Em = 535/587 nm)
  • Application: Measurement of Total Diamine Oxidase (DAO) activity in various biological samples.

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Species Reactivity

Mammalian/Bacterial

Applications

Measurement of Diamine Oxidase (DAO) in various biological samples.

Features and Benefits

  • Rapid & Convenient
  • The assay can detect as little as 1 µU of DAO enzymatic activity.
  • Includes DAO Enzyme as Positive Control

Kit Components

  • DAO Assay Buffer
  • DAO Probe (in DMSO)
  • DAO Substrate (Lyophilized)
  • DAO Enzyme Mix (Lyophilized)
  • H2O2 Standard (0.88 M)
  • DAO Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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