Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric) (BN00512)

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  • Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric) (BN00512)
  • Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric) (BN00512)


ELISA Kit Technical ManualMSDS

Dihydrofolate Reductase Inhibitor Screening Kit (Colorimetric)

Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC, is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells, playing a key role in folate metabolism. Inhibition of DHFR results in reduction of the intracellular level of tetrahydrofolate, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important therapeutic target for anti-tumor drugs. Methotrexate (MTX) is the most widely investigated inhibitor of DHFR, inhibiting both prokaryotic and eukaryotic DHFRs, and has shown antitumor activity. Assay Genie's Dihydrofolate Reductase Inhibitor Screening Kit is designed for screening DHFR inhibitors. MTX is used as a positive control. The DHFR activity is monitored by the reduction in absorbance reading at OD340 nm, while potential inhibitors arrest this decrease. The kit is adapted to a 96-well format and provides a rapid, simple, sensitive, and reliable test for high-throughput screening of DHFR inhibitors.

Figure: (a) Dihydrofolate Reductase activity with or without Methotrexate. (b) Inhibition of Dihydrofolate Reductase activity by Methotrexate (final conc.: 1 µM). IC 50 of Methotrexate was calculated to be 13.25 ±1.18 nM. Assay was carried out following the kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Absorbance (OD= 340 nm)

Species Reactivity



Screening/characterizing/studying ACE2 inhibitors/activators

Features and Benefits

  • Simple and reliable test to screen DHFR inhibitors/activators
  • High-throughput compatible
  • Includes Inhibitor Control (Methotrexate)

Kit Components

  • DHFR Assay Buffer
  • DHFR Substrate
  • Dihydrofolate Reductase
  • Methotrexate (10 mM)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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