DNAse I Activity Assay Kit (Fluorometric) (BN00674)

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SKU:
BN00674
  • DNAse I Activity Assay Kit (Fluorometric) (BN00674)
  • DNAse I Activity Assay Kit (Fluorometric) (BN00674)
  • DNAse I Activity Assay Kit (Fluorometric) (BN00674)
$872.80

Description

ELISA Kit Technical ManualMSDS

DNAse I Activity Assay Kit (Fluorometric)

Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5phosphorylated and 3hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. Assay Genie's DNAse I Activity Fluorometric Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per ml

Figure: a) DNA Probe to Product conversion standard curve; b) representative activity curve for purified DNAse I (orange), serum sample (green), and background control (blue) at 37°C; c) comparative analysis of DNAse I activity from 25 µl undiluted single donor normal vs. Systematic Lupus Erythematosus (SLE) patient serum sample.

Key Information Description

Product SKU

BN00674

Size

100 assays

Kit Summary

  • Detection method - Fluorescence (Ex/Em 651/681 nm)
  • Species reactivity - Eukaryotes
  • Applications - Measurement of DNAse activity in various biological samples.

Detection Method

Fluorescence (Ex/Em 651/681 nm)

Species Reactivity

Eukaryotes

Applications

  • Measurement of DNAse I activity of purified proteins
  • Quantitative analysis of DNAse I mutants and inhibitors Comparative examination of DNAse I activity in biological samples

Features and Benefits

Quantitative analysis of DNAse I mutants and inhibitors

Kit Components

  • 10X DNAse I Assay Buffer
  • DNA Probe
  • DNA Probe Re-suspension Buffer
  • DNAse I Positive Control
  • Positive Control Re-suspension Buffer
  • Molecular Biology Grade Water

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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