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Anti-DNMT1 Antibody (CAB15050)

SKU:
CAB15050
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€139 - €329
Frequently bought together:

Description

Antibody Name:Anti-DNMT1 Antibody (CAB15050)
Antibody SKU:CAB15050
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, Jurkat, U-87MG, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAR TAPA RVPT LAVP AISL PDDV RRRL KDLE RDSL TEKE CVKE KLNL LHEF LQTE IKNQ LCDL ETKL RKEE LSEE GYLA KVKS LLNK DLSL ENGA HAYN REVN GRLE NGNQ ARSE ARRV GMAD ANSP PKPL SKPR TPRR SKSD GEAK PEPS PSPR ITRK STRQ TTIT SHFA KGPA KRKP QEES ERAK SDES IKEE DKDQ DEKR RRVT SRER VARP LPAE EPER AKSG TRTE KEEE RDEK EEKR LRSQ TKEP TPKQ KLKE EPDR EARA GV
Cellular Location:Nucleus
Calculated MW:144kDa/183kDa/184kDa
Observed MW:200KDa
Synonyms:ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNMT1
Background:This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Western blot analysis of extracts of various cell lines, using DNMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using DNMT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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