E.Coli Dnak ATPase Binding Domain Recombinant Protein (CPRT5212)

SKU:
CPRT5212
€278

Description

Data SheetMSDS

DnaK ATPase-BD E.Coli Recombinant Protein

Name:DnaK ATPase-BD E.Coli
Synonyms:HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.
Introduction:DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial HSP–70 chaperone. This protein is involved in the foldingand assembly of newly synthesized polypeptide chains and in preventingthe aggregation of stress–denatured proteins. DnaK(amino acids1–384) is N–terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate bindingdomain (residues 385–638). The protein codingregion of the ATPase domain of DNAK (amino acids 1–384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was purified to apparent homogeneity by usingconventional column chromatography techniques.
Description:Recombinant DnaK Substrate BindingDomain produced in E.Coli is a single, non–glycosylated polypeptide chain containing 384 amino acids and having a molecular mass of 41.6 kDa.
Source:Escherichia Coli.
Pack size:50µg
Physical Appearance:Sterile filtered colorless solution.
Formulation:The DnaK protein contains 25mM Tris–HCl, pH7.5, 100mM NaCl, 5mM DTT and 10% Glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 95.0% as determined by SDS–PAGE.
Amino Acid Sequence:MGKIIGIDLgTTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVTNPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVLKKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYGLDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYLVEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKEPRKDVNPDEA VAIGAAVQGgVLTG.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
View AllClose

Additional Information

Product type:
Recombinant Protein
Reactivity:
E.Coli
Host Species:
E.Coli
View AllClose