|Product Name:||E.Coli Dnak SBD C-terminus Recombinant Protein|
|Target:||Dnak SBD C-terminus|
|Synonyms:||HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.|
|Physical Appearance:||Sterile filtered colorless solution.|
|Formulation:||The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10% Glycerol.|
|Stability:||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.|
|Purity:||Greater than 95.0% as determined by SDS-PAGE.|
|Amino Acid Sequence:||MDVK DVLL LDVT PLSL GIET MGGV MTTL IAKN TTIP TKHS QVFS TAED NQSA VTIH VLQG ERKR AADN KSLG QFNL DGIN PAPR GMPQ IEVT FDID ADGI LHVS AKDK NSGK EQKI TIKA SSGL NEDE IQKM VRDA EANA EADR KFEE LVQT RNQG DHLL HSTR KQVE EAGD KLPA DDKT AIES ALTA LETA LKGE DKAA IEAK MQEL AQVS QKLM EIAQ QQHA QQQT AGAD ASAN NAKD DDVV DAEF EEVK DKK|
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.The protein coding region of the substrate binding domain of DnaK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
Recombinant Dnak Substrate Binding Domain C-terminal produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids and having a molecular mass of 27.7 kDa.