|Product Name:||E.Coli hchA Recombinant Protein|
|Synonyms:||Chaperone protein hchA, EcHsp31, Hsp31, hchA, yedU, yzzC, b1967, JW1950.|
|Physical Appearance:||Sterile filtered colorless solution.|
|Formulation:||The hchA contains (1mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol 0.1M NaCl and 1mM DTT.|
|Stability:||hchA E.Coli Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.|
|Purity:||Greater than 95.0% as determined by SDS-PAGE.|
|Amino Acid Sequence:||MGSS HHHH HHSS GLVP RGSH MTV QTSK NPQV DIAE DNAF FPSE YSLS QYTS PVSD LDGV DYPK PYRG KHKI LVIA ADER YLPT DNGK LFST GNHP IETL LPLY HLHA AGFE FEVA TISG LMTK FEYW AMPH KDEK VMPF FEQH KSLF RNPK KLAD VVAS LNAD SEYA AIFV PGGH GALI GLPE SQDV AAAL QWAI KNDR FVIS LCHG PAAF LALR HGDN PLNG YSIC AFPD AADK QTPE IGYM PGHL TWYF GEEL KKMG MNII NDDI TGRV HKDR KLLT GDSP FAAN ALGK LAAQ EMLA AYAG|
Escherichia coli Hsp31 (HchA) is a homodimeric member of the ThiI/DJ-1/PfpI superfamily which combines molecular chaperone and aminopeptidase activities. HchA uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress.
hchA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-283 a.a.) and having a molecular mass of 33.3kDa.hchA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.