Ethanol Colorimetric/Fluorometric Assay Kit (BN00844)

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SKU:
BN00844
  • Ethanol Colorimetric/Fluorometric Assay Kit (BN00844)
  • Ethanol Colorimetric/Fluorometric Assay Kit (BN00844)
€643

Description

ELISA Kit Technical ManualMSDS

Ethanol Colorimetric/Fluorometric Assay Kit

Alcohol (ethanol CH) is among the most widely consumed drinks. Low doses of alcohol may help circulation while heavy alcohol consumption may lead to various forms of disease. Quantitative determination of alcohol finds applications in basic research, drug discovery, clinical studies and fermentation industry processes. Assay Genie's Ethanol Assay Kit provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media. Alcohol oxidase oxidizes ethanol to generate Hwhich reacts with our probe to generate color (ax= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit detects 0.1-10 ppm alcohol (~10-800 nM).

Figure: a) Ethanol Standard Curve. b) Measurement of Ethanol in fermented fruit extract (Cherry extract, 5 ul, 50X diluted). Fruit extract was treated with Carrez Clarification Reagent Kit (K809) for protein precipitation, spin filtered and diluted for the assay. Assay was performed following the kit protocol.

Key Information Description

Product SKU

BN00844

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species reactivity

Mammalian

Applications

The kit detects 0.1-10 ppm alcohol (~10-800 nM).

Features and Benefits

  • Simple procedure; takes ~40 minutes - 1 hour
  • Fast and convenient
  • Kit contains the necessary reagents for accurate measurement of ethanol in biological samples

Kit Components

  • Ethanol Assay Buffer
  • Ethanol Probe (in DMSO,anhydrous)
  • Ethanol Enzyme Mix (lyophilized)
  • Ethanol Standard (MW:46.07, 17.15N)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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