Exosome Isolation Kit (Cell Media) (BN00097)

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BN00097
  • Exosome Isolation Kit (Cell Media) (BN00097)
  • Exosome Isolation Kit (Cell Media) (BN00097)
  • Exosome Isolation Kit (Cell Media) (BN00097)
€756

Description

ELISA Kit Technical ManualMSDS

Exosome Isolation Kit (Cell Media)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of the released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Isolation Kit (Cell Media) is a fast and convenient method of exosome isolation and purification at high yields from cell culture media. This kit yields much highly pure exosomes as compared to exosome precipitation methods.

Figure: 1) Representative dynamic light scattering (DLS, 632.8 nm laser) number distribution measurement of isolated exosome population from NIH3T3 cells (1x10 6 cells) demonstrates a single peak (~150 nm) diameter. Homogenous Size Distribution of Exosomes Isolated by ExoGenie Kit comparable to exosomes isolated following ultracentrifugation protocols. 2) Analysis of exosomal RNA levels from NIH 3T3 cell media. Exosome Isolation kit produced greater quantity of exosomal RNA than ultracentrifugation as verified by exosomal RNA quantification.

Key Information Description

Applications

Exosome isolation and purification from cell culture media.

Size Options

  • 2 Reactions
  • 10 Reactions

Features and Benefits

Easy to use: No ultra-centrifugation (<2 hr)

Kit Components

  • Solution A (Blue)
  • Solution B
  • Solution C
  • Exosome Column

Storage Conditions

RT

Shipping Conditions

RT

USAGE

For Research Use Only! Not For Use in Humans.

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