Exosome Maxi Chromatography Columns (BN00121)

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BN00121
$1,683

Description

ELISA Kit Technical ManualMSDS

Exosome Maxi Chromatography Columns

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying exosomes and extracellular vesicles (EVs) from different matrices (urine and cell culture media).
ExoGenie Maxi columns allow the isolation of EVs from big volumes (up to 20 ml) and the column is particularly recommended for working with diluted matrices as urine or cell culture media.

Figure: PURE-EVs column was loaded with 20 ml of cell medium10 fold concentrated in MWCO concentrator 100K. 60 fractions (1ml each) have been collected and analyzed by ELISA ExoGenie assay and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 15 - 40 (turnaround time approximately 30 min), whereas plasma circulating proteins corresponded to the fractions 45 - 60.

Key Information Description

Kit Summary

  • For the isolation of highly pure exosomes from biofluids (plasma, serum, urine) and cell media
  • For EV purification of pre-isolated EVs from contaminants (100 µl up to 500 µl)
  • Easy and fast protocol (approximately 15 minutes)
  • Easy to store and ship at 4°C

Size Options

  • 3 Columns
  • 6 Columns

Applications

  • Extracellular vesicles isolation from diluted matrices (biofluids and cell media).
  • For EV purification of pre-isolated EVs from contaminants (5 ml up to 20 ml)
  • Isolated exosomes are suitable for multiple analysis including NTA, ELISA, FACS, WB, EM, MS, nucleic acid extraction etc.

Features and Benefits

  • Easy and fast protocol (approximately 15 minutes)
  • Isolate exosome from diluted matrices and different sample types
  • Easy to ship and store at 4°C

Kit Components

Maxi SEC Columns

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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