Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Mini Size Exclusion Chromatography (Mini SEC) columns is considered one of the best methods for isolating and purifying exosomes and extracellular vesicles (EVs) from small volume amount from plasma and serum or other biofluids. ExoGenie Mini Chromatography Columns is a SEC column designed for isolating EVs in a fast and easy way.

Figure: PURE-EVs column was rinsed with 200 μl of human plasma, 19 fractions (100 μl each one) have been collected and analyzed by ELISA ExoGenie assay and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 7 - 11 (turn around time approximately 15 min), whereas plasma circulating proteins corresponded to the fractions 13 - 19