FDG Beta-Galactosidase Assay Kit (CV0011)

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SKU:
CV0011
¥35,818.24

Description

ELISA Kit Technical ManualMSDS

FDG Beta-Galactosidase Assay Kit

FDG Beta-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active Beta-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the Beta-galactosidase is very resistant to proteolytic degradation and its activity is easily measured.

Beta-galactosidase performs the hydrolysis of fluorescein di-Beta-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of Beta-galactosidase is proportional to fluorescence produced.

Measure levels of active Beta-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Overview Description

Kit Size:

500 Assays

Features & Benefits:

  • Fast and convenient.
  • Easy-to-use method to quantify the enzyme expression in transfected cells.
  • Sensitive: measure Beta-galactosidase at femtogram level.

Kit Contents:

  • 55 mL Reaction Buffer
  • 25 mL Lysis Buffer
  • 25 mL Stop solution
  • 20 μL Beta-galactosidase enzyme (0.1 mg/mL)
  • 500 μL Beta-Mercaptoethanol
  • 125 μL FDG solution (10 mM)

Applications:

Measurement of Beta-Galactosidase activity in the lysates of transfected cell.

Shipping & Storage:

  • Shipped in: Gel Pack.
  • Storage: Upon receipt, store the kit at -20 ºC. Beta-galactosidase substrate must be protected from light.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Information:

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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