Flow Cytometric Apoptosis Detection Kit (BN00471)

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BN00471
€601

Description

ELISA Kit Technical ManualMSDS

Flow Cytometric Apoptosis Detection Kit

Activation of members of caspase-family proteases plays a key role in apoptosis. The Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry inLiving cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D), a reported substrate for members of caspase family proteases. The caspase substrate D is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

Key Information Description

Detection Method

Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).

Size Options

  • 25 Assays
  • 100 Assays

Species Reactivity

Mammalian

Applications

Detection of activated caspases in living cells.

Features and Benefits

  • Simple one-step procedure; takes only 30 minutes
  • Fast and convenient
  • D is more cell-permeable than other fluorometric caspase substrates

Kit Components

  • D Reagent
  • DTT (1 M)
  • D Incubation Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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