The General Carboxymethyl Lysine (CML) ELISA Kit is a powerful tool for the accurate measurement of CML levels in biological samples such as serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CML is a modified amino acid that is formed during the Maillard reaction, a process that occurs during the aging and cooking of food. Elevated levels of CML have been linked to various age-related diseases such as diabetes, cardiovascular disease, and neurodegenerative disorders.
By measuring CML levels accurately, researchers can gain valuable insights into the role of this compound in disease progression and potentially develop new therapeutic strategies.Overall, the General Carboxymethyl Lysine (CML) ELISA Kit is a valuable tool for researchers studying age-related diseases and looking to understand the role of CML in disease development and progression. Its high sensitivity and specificity make it an essential component of any research laboratory focused on uncovering novel therapeutic targets.
Product Name:
General Carboxymethyl Lysine (CML) ELISA Kit
Product Code:
UNDL00020
Size:
96 Assays
Target:
CML
Synonyms:
N(6)-Carboxymethyllysine
Detection Method:
Competition
Range:
78.12-5000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
