Glucose Dehydrogenase Activity Colorimetric Assay Kit (BN00999)

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BN00999
  • Glucose Dehydrogenase Activity Colorimetric Assay Kit (BN00999)
  • Glucose Dehydrogenase Activity Colorimetric Assay Kit (BN00999)
€511

Description

ELISA Kit Technical ManualMSDS

Glucose Dehydrogenase Activity Colorimetric Assay Kit

Glucose 1-dehydrogenase (NAD (EC 1.1.1.118 ) is an enzyme that catalyzes the chemical reaction: D-glucose + NADD-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NADor NADPas acceptor. Assay Genie's Glucose Dehydrogenase (GDH) Assay Kit provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (= 450 nm). This assay detects GDH activity as low as 0.01 mU with our unit definition.

                  (a)

(b)

Figure: (a) NADH Standard curve obtained using kit protocol. (b) Sample test curve *Sample: Bovine Liver extraction (36µg protein)

Key Information Description

Product SKU

BN00999

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

This assay detects GDH activity as low as 0.01 mU with our unit definition.

Features and Benefits

Sensitive assays for measuring Glucose Dehydrogenase in various biological samples

Kit Components

  • GDH Assay Buffer
  • Glucose (2 M)
  • Developer (Lyophilized)
  • GDH Positive Control (Lyophilized)
  • NADH Standard (0.5 ol, Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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