Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. Assay Genie's GDH Assay Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (= 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

Figure: NADH Standard Curve obtained using kit protocol and GDH timeline graph. *Sample 01: Bovine Liver extraction (2 µg protein), Sample 02: 5 µl Rabbit serum