Glutathione Colorimetric Assay Kit (BN00529)

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SKU:
BN00529
  • Glutathione Colorimetric Assay Kit (BN00529)
  • Glutathione Colorimetric Assay Kit (BN00529)
  • Glutathione Colorimetric Assay Kit (BN00529)
€589

Description

ELISA Kit Technical ManualMSDS

Glutathione Colorimetric Assay Kit

Glutathione (GSH) is the major intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative stress in mammalian cells. Assay Genie's Glutathione Colorimetric Assay Kit provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader. The assay is based on the glutathione recycling system by DTNB and glutathione reductase (Fig. 1). DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection. The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and glutamyl transpeptidase reaction. The kit can quantify glutathione from 1-100 ng/well in a 200 reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutahione.

Glutathione Standard Curve: Various amounts of standard glutathione was added to the glutathione reaction and incubated for 10 min according to the kit instructions. Absorbance was measured at O.D. 405 nm.

Key Information Description

Product SKU

BN00529

Size

100 assays

Detection Method

Absorbance (412 nm)

Species Reactivity

Mammalian

Applications

The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes

Features and Benefits

  • Simple procedure; takes only ~2-3 hours
  • Fast and convenient

Kit Components

  • Glutathione Reaction Buffer
  • Glutathione Substrate (DTNB)
  • NADPH Generating Mix (lyophilized)
  • Glutathione Reductase (lyophilized)
  • Sulfosalicylic Acid (SSA, 1 gram)
  • GSH Standard (lyophilized, MW 307)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans

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