Glutathione Fluorometric Assay Kit (BN00517)

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SKU:
BN00517
€553

Description

ELISA Kit Technical ManualMSDS

Glutathione Fluorometric Assay Kit

Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. Assay Genie's Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.

Figure: General Standard Curve Obtained using Kit Protocol.

Key Information Description

Product SKU

BN00517

Size

100 assays

Detection Method

Fluorescence (Ex/Em 380/461 nm)

Species Reactivity

Mammalian

Applications

The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.

Features and Benefits

  • Simple two-step procedure; takes only 2 hours
  • Fast and convenient
  • The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione.

Kit Components

  • Cell Lysis Buffer
  • Monochlorobimane
  • GST Reagent
  • GSH Standard (1 mg; MW: 307)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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