Description
Glutathione Reductase Activity Colorimetric Assay Kit
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Assay Genie's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, ax = 405 nm). The assay can detect 0.1 - 40 mU/ml GR in various samples.
Figure: (a) Standard Curve Obtained using Kit Protocol, (b) Glutathione Reductase Activity graph.
Key Information | Description |
Product SKU | BN00974 |
Size | 200 assays |
Detection Method | Absorbance (405 nm) |
Species Reactivity | Mammalian |
Applications | The assay can detect 0.1 - 40 mU/ml GR in various samples. |
Features and Benefits |
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Kit Components |
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Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |