Glutathione Reductase Activity Colorimetric Assay Kit (BN00974)

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BN00974
  • Glutathione Reductase Activity Colorimetric Assay Kit (BN00974)
  • Glutathione Reductase Activity Colorimetric Assay Kit (BN00974)
€589

Description

ELISA Kit Technical ManualMSDS

Glutathione Reductase Activity Colorimetric Assay Kit

Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Assay Genie's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, ax = 405 nm). The assay can detect 0.1 - 40 mU/ml GR in various samples.


Figure: (a) Standard Curve Obtained using Kit Protocol, (b) Glutathione Reductase Activity graph.

Key Information Description

Product SKU

BN00974

Size

200 assays

Detection Method

Absorbance (405 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 0.1 - 40 mU/ml GR in various samples.

Features and Benefits

  • Simple procedure; takes ~ less than 40 minutes
  • Fast and convenient

Kit Components

  • GR Assay Buffer
  • 3% Hbr> Catalase
  • TNB Standard (2.5 µmol)
  • DTNB
  • NADPH
  • GSSG
  • GR Positive Control (10 mU)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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