Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. Assay Genie's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, ax = 405 nm). The assay can detect 0.1 - 40 mU/ml GR in various samples.

Figure: (a) Standard Curve Obtained using Kit Protocol, (b) Glutathione Reductase Activity graph.