Glycine Assay Kit (Fluorometric) (BN00817)

(No reviews yet) Write a Review
SKU:
BN00817
  • Glycine Assay Kit (Fluorometric) (BN00817)
  • Glycine Assay Kit (Fluorometric) (BN00817)
€786

Description

ELISA Kit Technical ManualMSDS

Glycine Assay Kit (Fluorometric)

Glycine (GLY) is one of the 20 standard amino acids commonly found in proteins. GLY side chain is a hydrogen substituent, thus it is the only amino acid that is not chiral and the smallest among the proteogenic amino acids. Basic functions of Glycine include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also present as one of the most abundant residues in the triple-helical structure of collagen. It acts as a glucogenic amino acid regulating sugar levels in blood. Therefore, glycine supplementation has been used in patients suffering anemia, hypoglycemia and chronic fatigue. In cancer cells, GLY consumption is highly correlated to cancer cell proliferation, via purine synthesis. Glycine uptake in cancer cell studies supports the role of this amino acid in tumorigenesis and malignancy. GLY possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. Assay Genie's Glycine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of glycine in a variety of biological fluids. The principle of the assay is based on the oxidation of glycine producing a fluorophore (Ex/Em = 535/587 nm) with a stable signal, which is directly proportional to the amount of GLY in the sample. The assay is specific and other standard and non-standard amino acids do not interfere with the assay. The assay can detect as little as 1 µM of Glycine in a variety of samples.

Figure: (a) Glycine Standard Curve. (b) Estimation of Glycine concentration in human serum, saliva, and urine. Samples were deproteinized using 10 kDa spin colum and diluted using GLY Assay Buffer (Serum: 64-fold; Saliva: 32-fold; Urine: 128-fold). 25 µl of each diluted sample was spiked with 0.3 nmol of Glycine Standard and assayed following the kit protocol. Glycine concentrations are: Serum: 224 ± 21 µM, Saliva: 149 ± 7 µM, Urine: 54 ±4 µM/mM Creatinine.

Key Information Description

Product SKU

BN00817

Size

100 assays

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

Estimation of glycine in various biological samples

Features and Benefits

  • Simple, rapid & convenient assay
  • Limit of detection: 1 µM
  • Very specific, does not interact with other standard and non-standard amino acids

Kit Components

  • GLY Assay Buffer
  • GLY Probe
  • GLY Enzyme Mix
  • GLY Developer
  • GLY Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose