Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of 1,4 linkage with 1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. Assay Genie's Glycogen Assay kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect less than 4 µg/ml of Glycogen in samples.

Figure: (a) Glycogen Standard curve. (b) Measurement of glycogen levels in rat Liver (20 µg) and rat muscle (40 µg). Assays were performed following Kit protocol.