Glycogen Colorimetric Assay Kit II (BN00872)

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SKU:
BN00872
  • Glycogen Colorimetric Assay Kit II (BN00872)
  • Glycogen Colorimetric Assay Kit II (BN00872)
€708

Description

ELISA Kit Technical ManualMSDS

Glycogen Colorimetric Assay Kit II

Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of 1,4 linkage with 1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. Assay Genie's Glycogen Assay kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect less than 4 µg/ml of Glycogen in samples.

Figure: (a) Glycogen Standard curve. (b) Measurement of glycogen levels in rat Liver (20 µg) and rat muscle (40 µg). Assays were performed following Kit protocol.

Key Information Description

Product SKU

BN00872

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

  • The assay can detect less than 4 µg/ml of Glycogen in various tissues.
  • Analysis of metabolism and cell signaling.

Features and Benefits

  • Simple, rapid, and high-throughput adaptable.
  • Kit contains all necessary reagents for accurate measurement of Glycogen levels.

Kit Components

  • Glycogen Hydrolysis Buffer
  • Gycogen Development Buffer
  • Hydrolysis Enzyme Mix (lyophilized)
  • Development Enzyme Mix (lyophilized)
  • Probe (lyophilized)
  • Glycogen Standard (2 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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