GNAS Antibody (PACO57068)
- SKU:
- PACO57068
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GNAS Antibody (PACO57068) |
| Antibody SKU: | PACO57068 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein ALEX protein (310-404AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: GNAS antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68 kDa. Observed band size: 68 kDa. |
 | IHC image of PACO57068 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of MCF-7 cells with PACO57068 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | May inhibit the adenylyl cyclase-stimulating activity of guanine nucleotide-binding protein G(s) subunit α which is produced from the same locus in a different open reading frame. |
| Synonyms: | Protein ALEX (Alternative gene product encoded by XL-exon), GNAS, GNAS1 |
| UniProt Protein Function: | G-alpha(s) iso7: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. |
| UniProt Protein Details: | Protein type:Oncoprotein; G protein; G protein, heterotrimeric alpha G(s); Membrane protein, peripheral; G protein, heterotrimeric; Vesicle Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; dendrite; extracellular region; plasma membrane; heterotrimeric G-protein complex; trans-Golgi network membrane; intrinsic to membrane; cytosol; nucleus Molecular Function:corticotropin-releasing hormone receptor 1 binding; GTPase activity; GTP binding; metal ion binding; adenylate cyclase activity; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; beta-2 adrenergic receptor binding; G-protein beta/gamma-subunit binding; D1 dopamine receptor binding Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; female pregnancy; embryonic hindlimb morphogenesis; G-protein signaling, adenylate cyclase activating pathway; intracellular transport; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; transmembrane transport; post-embryonic body morphogenesis; endochondral ossification; dopamine receptor, adenylate cyclase activating pathway; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; sensory perception of smell; adenylate cyclase activation; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; blood coagulation; cognition; regulation of insulin secretion Disease: Osseous Heteroplasia, Progressive; Pseudopseudohypoparathyroidism; Pituitary Adenoma, Acth-secreting; Mccune-albright Syndrome; Acth-independent Macronodular Adrenal Hyperplasia; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pituitary Adenoma, Growth Hormone-secreting |
| NCBI Summary: | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012] |
| UniProt Code: | P84996 |
| NCBI GenInfo Identifier: | 7532780 |
| NCBI Gene ID: | 2778 |
| NCBI Accession: | AAF63226.1 |
| UniProt Secondary Accession: | P84996,O95417, B2RB88, E1P5G2, |
| UniProt Related Accession: | O95467,P63092,P84996,Q5JWF2 |
| Molecular Weight: | 111,025 Da |
| NCBI Full Name: | neuroendocrine secretory protein 55 |
| NCBI Synonym Full Names: | GNAS complex locus |
| NCBI Official Symbol: | GNASÂ Â |
| NCBI Official Synonym Symbols: | AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45Â Â |
| NCBI Protein Information: | protein ALEX; protein GNAS; protein NESP55; secretogranin VI; extra large alphas protein; neuroendocrine secretory protein; guanine nucleotide regulatory protein; alternative gene product encoded by XL-exon; adenylate cyclase-stimulating G alpha protein; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 |
| UniProt Protein Name: | Neuroendocrine secretory protein 55 |
| Protein Family: | Neuroendocrine secretory protein |
| UniProt Gene Name: | GNASÂ Â |
| UniProt Entry Name: | GNAS3_HUMAN |
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