GSH Assay Kit (Colorimetric) (BN00700)

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SKU:
BN00700
  • GSH Assay Kit (Colorimetric) (BN00700)
  • GSH Assay Kit (Colorimetric) (BN00700)
  • GSH Assay Kit (Colorimetric) (BN00700)
  • GSH Assay Kit (Colorimetric) (BN00700)
€631

Description

ELISA Kit Technical ManualMSDS

GSH Assay Kit (Colorimetric)

Glutathione (GSH), a thiol-containing tripeptide (glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges. Assay Genie's Reduced Glutathione Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at 450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. Our kit is the most specific and sensitive quantitative assay in the market because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples' GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.

Figure: (a-b) GSH Standard Curve, results from multiple experiments. (c) Assay Specificity. Measurement of GSH (1 nmol) and GSSG (100 nmol). The assay kit can effectively discriminate between reduced GSH and oxidized GSSG forms. (d) Measurement of GSH in Mouse Liver (10 µg protein), Hep G2 Cell Extract (6 µg protein) and Jurkat Cell Extrac (10 µg protein). All assays were performed following kit protocol.

Key Information Description

Product SKU

BN00700

Size

100 assays

Kit Components

  • Detection method- Absorbance (450 nm)
  • Species reactivity- Mammalian
  • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Detection Method

Detection method- Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Features and Benefits

  • Simple procedure; takes only ~1-2 hours
  • Fast and convenient
  • Specific: does not detect oxidized GSSG
  • Sensitive: detects as low as 50 pmol GSH

Kit Components

  • GSH Assay Buffer
  • Substrate Mix A
  • Substrate Mix B
  • Enzyme Mix A
  • Enzyme Mix B
  • Enzyme Mix C
  • Sulfosalicylic Acid (SSA, 1 gram)
  • GSH Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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