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Anti-HAS2 Antibody (CAB9897)

SKU:
CAB9897
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-HAS2 Antibody
Antibody SKU:CAB9897
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 67-185 of human HAS2 (NP_005319.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:SW620, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 67-185 of human HAS2 (NP_005319.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EHRK MKKS LETP IKLN KTVA LCIA AYQE DPDY LRKC LQSV KRLT YPGI KVVM VIDG NSED DLYM MDIF SEVM GRDK SATY IWKN NFHE KGPG ETDE SHKE SSQH VTQL VLSN KSIC IMQ
Gene ID:3037
Uniprot:Q92819
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:63kDa
Observed MW:70kDa
Synonyms:HAS2
Background: Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.
UniProt Protein Function:HAS2: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Expressed in fibroblasts. Belongs to the nodC/HAS family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, multi-pass; Transferase; EC 2.4.1.212; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: integral to plasma membrane

Molecular Function:hyaluronan synthase activity

Biological Process: extracellular polysaccharide biosynthetic process; hyaluronan biosynthetic process; positive regulation of cell proliferation; kidney development; vasculogenesis; positive regulation of cell migration

NCBI Summary:Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]
UniProt Code:Q92819
NCBI GenInfo Identifier:4885391
NCBI Gene ID:3037
NCBI Accession:NP_005319.1
UniProt Secondary Accession:Q92819,Q32MM3,
UniProt Related Accession:Q92819
Molecular Weight:63566
NCBI Full Name:hyaluronan synthase 2
NCBI Synonym Full Names:hyaluronan synthase 2
NCBI Official Symbol:HAS2  
NCBI Protein Information:hyaluronan synthase 2
UniProt Protein Name:Hyaluronan synthase 2
UniProt Synonym Protein Names:Hyaluronate synthase 2; Hyaluronic acid synthase 2; HA synthase 2
Protein Family:Hyaluronan synthase
UniProt Gene Name:HAS2  
UniProt Entry Name:HYAS2_HUMAN
Anti-HAS2 Polyclonal Antibody (CAB9897)Western blot analysis of extracts of various cell lines, using HAS2 antibody (CAB9897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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