HDAC Activity Fluorometric Assay Kit (BN00597)

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SKU:
BN00597
€565

Description

ELISA Kit Technical ManualMSDS

HDAC Activity Fluorometric Assay Kit

Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and induce apoptosis or differentiation in cancer cells. However, screening compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation of the substrate sensitizes the substrate, so that further treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications. HDAC inhibitors and antibodies are also available separately.

Figure: Analyses of HDAC Activity in HeLa Nuclear Extract. HeLa nuclear extract (NE) in various amounts were incubated with 5 µl HDAC fluorometric substrate. After 30 min, the reactions were stopped with 10 µl Lysine Developer. Samples were then read in a fluorescence plate reader with Ex/Em = 360/460 nm.

Key Information Description

Product SKU

BN00597

Size

100 assays

Detection Method

Fluorescence (Ex/Em 350-380/440-460 nm)

Species Reactivity

Mammalian

Applications

  • Well suited for high throughput applications.
  • HDAC inhibitors and substrates and related antibodies are also available separately.

Features and Benefits

  • Simple two-step procedure; takes around than 1 hour
  • Fast and convenient
  • The assay method eliminates radioactivity/extractions/ and/or chromatography as used in the traditional assays.

Kit Components

  • HDAC Substrate [Boc-Lys(Ac)-AMC, 4 mM]
  • 10X HDAC Assay Buffer
  • Lysine Developer
  • HDAC Inhibitor (Trichostatin A, 1 mM)
  • HeLa Nuclear Extract (5 mg/ml)
  • Deacetylated Standard [Boc-Lys-AMC, 4 mM]

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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