Anti-HDAC10 Antibody (CAB6184)

Antibody Name:	Anti-HDAC10 Antibody
Antibody SKU:	CAB6184
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDAC10 (NP_114408.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HL-60, 293T, HeLa, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDAC10 (NP_114408.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGTA LVYH EDMT ATRL LWDD PECE IERP ERLT AALD RLRQ RGLE QRCL RLSA REAS EEEL GLVH SPEY VSLV RETQ VLGK EELQ ALSG QFDA IYFH PSTF HCAR LAAG AGLQ LVDA VLTG AVQN GLAL VRPP GHHG QRAA ANGF CVFN NVAI AAAH AKQK HGLH RILV VDWD VHHG QGIQ YLFE DDPS VLYF SWHR YEHG RFWP FLRE SDAD AVGR GQGL GFTV NLPW NQVG MGNA DYVA
Gene ID:	83933
Uniprot:	Q969S8
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	43kDa/69kDa/70kDa/71kDa
Observed MW:	68kDa

Synonyms:

HDAC10, HD10

Background:

The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HDAC10: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Belongs to the histone deacetylase family. HD type 2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Deacetylase; EC 3.5.1.98 Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nucleus Molecular Function:NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity Biological Process: establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein amino acid deacetylation; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte development; negative regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q969S8
NCBI GenInfo Identifier:	27734403
NCBI Gene ID:	83933
NCBI Accession:	Q969S8.1
UniProt Secondary Accession:	Q969S8,Q08AP4, Q6STF9, Q96P77, Q96P78, Q9H028, Q9UGX1 Q9UGX2,
UniProt Related Accession:	Q969S8
Molecular Weight:	669
NCBI Full Name:	Histone deacetylase 10
NCBI Synonym Full Names:	histone deacetylase 10
NCBI Official Symbol:	HDAC10
NCBI Official Synonym Symbols:	HD10
NCBI Protein Information:	histone deacetylase 10
UniProt Protein Name:	Histone deacetylase 10
Protein Family:	Histone deacetylase
UniProt Gene Name:	HDAC10
UniProt Entry Name:	HDA10_HUMAN

	Western blot analysis of extracts of various cell lines, using HDAC10 antibody (CAB6184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of rat liver, using HDAC10 antibody (CAB6184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies	ELISA Kits
HDAC10 Antibody (PACO00909)	HDAC10 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)