HDAC3 Inhibitor Screening Kit (Fluorometric) (BN00626)

  • Cell Biology Assays HDAC3 Inhibitor Screening Kit Fluorometric BN00626
  • Cell Biology Assays HDAC3 Inhibitor Screening Kit Fluorometric BN00626


ELISA Kit Technical ManualMSDS

HDAC3 Inhibitor Screening Kit (Fluorometric)

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In Assay Genie's HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.

Figure: (a) TSA Inhibition of HDAC3 Activity (b) Typical Response of HDAC3 to Trichostain A (1.25µM)

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Em/Ex = 380/500 nm)


Suitable for high throughput screening of HDAC3 inhibitorsv

Features and Benefits

  • Simple procedure; takes ~ 60 min
  • Fast and convenient
  • Kit contains the necessary reagents for testing putative HDAC3 inhibitors

Kit Components

  • HDAC3 Assay Buffer
  • HDAC3 Substrate
  • HDAC-3 Enzyme
  • Developer
  • Trichostatin A

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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