HDAC8 Inhibitor Screening Kit (Fluorometric) (BN00631)

SKU:
BN00631
Product type:
Cellular Assay
  • Cell Biology Assays HDAC8 Inhibitor Screening Kit Fluorometric BN00631
  • Cell Biology Assays HDAC8 Inhibitor Screening Kit Fluorometric BN00631
€739

Description

ELISA Kit Technical ManualMSDS

HDAC8 Inhibitor Screening Kit (Fluorometric)

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In Assay Genie's HDAC-8 Inhibitor screening Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. In the presence of an HDAC-8 inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release AFC. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-8 inhibitors. Trichostatin A is included as a control inhibitor to compare the efficacy of the test inhibitors.

Figure: Standard Curves obtained using kit protocol.

Key Information Description

Product SKU

BN00631

Size

100 assays

Detection Method

Fluorescence (Em/Ex = 380/500 nm)

Applications

Suitable for high throughput screening of HDAC-8 inhibitors

Features and Benefits

  • Simple procedure; takes ~ 60 min
  • Fast and convenient
  • Kit contains the necessary reagents for testing putative HDAC-8 inhibitors

Kit Components

  • HDAC-8 Assay Buffer
  • HDAC-8 Substrate
  • HDAC-8 Enzyme
  • Developer
  • Trichostatin A (20 µM in DMSO)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans