Hemoglobin Colorimetric Assay Kit (BN00496)

  • Cell Biology Assays Hemoglobin Colorimetric Assay Kit BN00496
  • Cell Biology Assays Hemoglobin Colorimetric Assay Kit BN00496


ELISA Kit Technical ManualMSDS

Hemoglobin Colorimetric Assay Kit

Hemoglobin (Hb) is an iron containing globular metalloprotein found primarily in red blood cells. It carries oxygen from the lungs to the rest of the body as oxyhemoglobin and then returns back to the lungs as deoxyhemoglobin. In most vertebrates, hemoglobin is in tetrameric form comprising two globin chains and two Beta-globin chains (Beta2). Each chain is associated with a non-protein heme group. Aside from oxygen transport, hemoglobin can bind and transport other molecules like nitric oxide and carbon monoxide. Hemoglobin also plays an important role in maintaining the shape of the red blood cells. Alterations in the structure and binding capacity of hemoglobin are associated with many blood disorders such as sickle-cell anemia and thalassemia. Assay Genie's Hemoglobin Colorimetric Assay kit provides a quick and easy method for monitoring hemoglobin levels in a wide variety of samples. In this assay, the detector selectively converts heme into a stable chemical complex that absorbs maximally at 575 nm. The intensity of the color is directly proportional to the total concentration of hemoglobin in the sample. The kit can detect as low as 0.02 g/dl hemoglobin


Figure: (a) Hemoglobin Standard Curve (0-250 mg/dl). (b) Estimation of hemoglobin concentration in human plasma, serum, adult
male, adult female and sickle-cell anemia patient. Whole blood samples were diluted 10-fold. Assays were performed in triplicate following the kit protocol using 20 µl of the samples.

Key Information Description

Product SKU



200 assays

Detection Method

Absorbance (OD = 575 nm)

Species Reactivity



Estimation of Hemoglobin in various Biological Samples

Features and Benefits

  • Simple one-step procedure; takes only 15 minutes
  • Fast and convenient
  • Detects physiological concentrations of hemoglobins in human samples HTP adaptable

Kit Components

  • Hemoglobin Detector
  • Hemoglobin Standard (equivalent to 1 g/dl)

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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